1547 Characterization of a rat model with typical cutaneous small-vessel vasculitis induced by combined injection of an anti-phosphatidylserine/prothrombin complex antibody and an anti-LAMP-2 antibody

We previously reported that cutaneous vasculitis could be associated with anti-PS/PT antibody and LAMP-2. The copy number of LAMP-2 mRNAs in skin samples from rats induced by intravenous administration after subcutaneous histone injection was significantly higher than without vasculitis. Male Wistar-Kyoto (n=14) were given a dorsal cell-free histones then divided into four groups. Two hours later, Group 1 (n=4) intravenously administered rat IgM class monoclonal an anti-LAMP-2 polyclonal antibody. 2 (n=3) rabbit IgG instead the antibody, 3 4 followed used as another control. detected typical leukocytoclastic where neutrophils predominantly infiltrated nuclear dust scattered around blood vessels dermis all rats. These observations not evident systemic organs other skin. infiltration MPO-positive vasculitic lesions most abundant using immunofluorescent staining. observed developed lymphocyte-predominant infiltration. demonstrated neutrophil recruitment small results suggest introduction local serum moves to cell surface vascular endothelial cells, can bridge those cells through antigen-specific binding small-vessel